Skip to main content
. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: J Allergy Clin Immunol. 2021 Aug 21;149(3):1044–1059.e5. doi: 10.1016/j.jaci.2021.07.041

Figure 7.

Figure 7.

IFN-β inhibits GATA3 expression induced by STAT5-activating cytokines in lung ILC2s. (A) Isolated lung ILC2s were cultured with medium alone, IL-7, IL-2, TSLP or IL-33 for 72 h, and GATA3 protein expression in live ILC2s (i.e., Ghost Dye Red 780-negative) was analyzed. Representative histograms and scattergrams are shown. (B) MFI for GATA3 protein. (C) Proportion of GATA3-negative cells among the live cells. Data are presented as the mean ± SEM (n = 2). *p<0.05, **p<0.01 compared with cells cultured with medium alone. (D) Isolated lung ILC2s were cultured with medium alone or IL-7 with or without IFN-β or IFN-γ for 72 h, and GATA3 protein expression in live ILC2s (i.e., Ghost Dye Red 780-negative) was analyzed. (E) MFI for GATA3 protein. (F) Proportion of GATA3-negative cells among the live cells. Data are presented as the mean ± SEM (n = 3) and are representative of two experiments. *p<0.05, **p<0.01 between the groups indicated by horizontal lines.