Table 1.

CD8⁺ cytotoxic T lymphocytes (CTLs) epitope selection for PIM1, CDCP1, MMP14, MSLN, TROP2, and NECT4

Proteins	Epitopes	Positions		MHC class I alleles	IC50 values	Percentile rank	Antigenicity score
PIM1	DLFDFITER	128–136		HLA-A*68:01	8.76	0.07	1.1942
				HLA-A*33:01	12.09	0.02
				HLA-A*33:03	23.33	0.07
	KINSLAHLR	5–13		HLA-A*31:01	6.96	0.03	1.851
	KLIDFGSGA	183–191		HLA-A*02:06	24.44	0.23	1.3963
				HLA-A*02:01	37.53	0.34
	LILERPEPV	118–126		HLA-A*02:06	21.66	0.2	1.1386
				HLA-A*02:01	37.83	0.34
	MLLSKINSL	1–9		HLA-B*08:01	6.83	0.02	1.3426
				HLA-A*02:01	10.89	0.1
				HLA-A*02:06	27.02	0.25
CDCP1	KTISCTDHR	413–421		HLA-A*31:01	30.27	0.22	1.0479
	RIKMQEGVK	185–193		HLA-A*30:01	35.32	0.17	1.0898
	KVYLRTPNW	556–564		HLA-A*32:01	13.87	0.02	1.1621
				HLA-B*57:01	24.08	0.06
				HLA-B*58:01	31.67	0.15
	LLAKPCYIV	51–59		HLA-A*02:01	45.46	0.4	1.4682
	LPRESNITV	34–42		HLA-B*07:02	18.58	0.06	1.3577
MMP14	RFREVPYAY	158–166		HLA-C*14:02	22.21	0.1	1.0207
				HLA-A*30:02	37.07	0.05
				HLA-A*29:02	37.69	0.11
	PPRCLLLPL	7–15		HLA-B*07:02	34.05	0.1	1.277
	NYTPKVGEY	130–138		HLA-C*14:02	41.84	0.17	1.6215
MSLN	DLATFMKLR	527–535		HLA-A*68:01	25.71	0.28	1.0888
				HLA-A*33:01	26.12	0.04
				HLA-A*33:03	44.4	0.14
	SVSTMDALR	241–249		HLA-A*68:01	6.14	0.04	1.1239
				HLA-A*33:03	46.95	0.15
	FYPGYLCSL	444–452		HLA-C*14:02	4.28	0.02	1.2151
				HLA-C*07:02	28.76	0.01
	STMDALRGL	243–251		HLA-A*68:02	28.66	0.18	1.3541
				HLA-A*02:06	28.78	0.26
	TQMDRVNAI	334–342		HLA-A*02:06	11.31	0.11	1.7285
NECT4	QRITHILHV	234–242		HLA-C*06:02	31.33	0.02	1.0053
	GSVAEMSSY	305–313		HLA-C*03:02	40.14	0.17	1.0168
	AVTSEFHLV	203–211		HLA-A*02:06	26.51	0.25	1.0379
	GTTSSRSFK	189–197		HLA-A*11:01	17.98	0.08	1.2842
				HLA-A*30:01	29.54	0.13
	SSRDSQVTV	322–330		HLA-A*30:01	34.49	0.16	1.555
	EVKGTTSSR	186–194		HLA-A*68:01	21.45	0.22	1.9232
				HLA-A*33:03	40.26	0.13
TROP2	YLDEIPPKF	260	268	HLA-C*05:01	14.66	0.02	1.0799