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. 2022 Feb 7;12:790263. doi: 10.3389/fmicb.2021.790263

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Copyright © 2022 Migliaccio, Esposito, Bagattini, Berisio, Triassi, De Gregorio and Zarrilli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RT-qPCR assay of adeB, adeR, adeS, adeG, adeJ, amvA, craA, aceI, abeS, and abeM expression in the presence of LB and 4 mg/L and 8 mg/L CHX. Relative number of transcripts of each gene was normalized in each condition and calculated using the 2^–^ΔΔ^ct method compared to the expression level in LB control. The mean + standard deviation of relative number of transcripts is shown for each gene. All experiments were performed in triplicate. p-values were calculated using ANOVA (*p < 0.05; **p < 0.01; ***p < 0.001).