FIGURE 5.

RT-qPCR assay of amvA, aceI, adeB, and adeJ genes expression in the absence (LB) or presence of 4 mg/L CHX alone or in combination with 32 mg/L PIP (A) or 32 mg/L RV (B). Relative number of transcripts of each gene was normalized in each condition and calculated using the 2^–ΔΔct method compared to the expression level in LB control. The mean ± standard deviation of relative number of transcripts is shown for each gene. All experiments were performed in triplicate. P-values were calculated using ANOVA (*p < 0.01 vs. LB;°p < 0.01 vs. 4 mg/L CHX).