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. 2022 Feb 7;10:786052. doi: 10.3389/fcell.2022.786052

FIGURE 3.

FIGURE 3

The effects of expressing NC-enriched transcription factors in ectopic domains. (A) Ectopic expression of Foxd3 or Msx1(NC TFs) in a neural plate precursor blastomere resulted in reduced expression of foxd4 (NP TF) only in the region of the lineage-labeled cells (red dots, arrow). Vegetal views at midgastrula (st 11.5) with dorsal to the top. (B) The percentage of embryos in which the neural plate expression of foxd4 or sox2 were reduced by ectopic expression of either Foxd3 or Msx1. Numbers within the bars denote sample size. (C) Ectopic expression of Zic1 (NC TF) in a placode precursor blastomere reduced the PPR expression of six1 (arrow) in cells expressing Zic1 (red dots). Anterior view with dorsal to the top. (D) The percentage of embryos in which the PPR expression of six1 was reduced by ectopic expression of four different NC TFs. Numbers within the bars denote sample size. (E) Ectopic expression of Msx1 (NC TF) in an epidermis precursor blastomere reduced the Epi expression of epiker (between arrows) only in cells ectopically expressing Msx1 (red dots). Ventral view of gastrula stage with dorsal to the top. (F) Ectopic expression of Zic2 (NC TF) in an epidermis precursor blastomere reduced the Epi expression of epiker (between arrows) only in cells ectopically expressing Zic2 (red dots). Dorsal view of neurula stage with anterior to the top. (G) The percentage of embryos in which the expression of several Epi genes was reduced by ectopic expression of Msx1. Numbers within the bars denote sample size.