Figure 2.

Tofacitinib decreases the cytotoxicity of NK-92 to motor neurons in an in vitro ALS model. iNeurons were differentiated from control- or ALS-participant derived iPSCs and were co-cultured for four hours with pretreated NK-92 cells (IL-15 ± tofacitinib); cell death was quantitated by flow cytometry. (A) Gating strategy for quantitating cell death of iNeurons cultured alone, of NK-92 cells cultured alone, and iNeuron and NK-92 cell co-culture. Dead iNeurons were characterized by positive fluorescence levels of annexin V (apoptosis) and moderate levels of 7AAD viability dye (cell death). (B) The rate of iNeuron death was quantitated in control- and ALS-derived iNeurons following co-culture with NK-92 cells; data were normalized to cell death rates from co-culture with Unstimulated NK-92 cells; n = 6 independent experiments. Comparisons were by paired t-test to assessed paired, non-normally distributed data. *P < 0.05.