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. 2022 Feb 7;12:796715. doi: 10.3389/fmicb.2021.796715

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Copyright © 2022 Chen, Xie, Chan and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Research design for resolution of the plasmid profile of Salmonella strain Sa42 and the corresponding transconjugants by S1-PFGE. (A) Plasmids pBackZero-ISEcp1 and pBackZero-IS15DI were transferred to (B) strain Sa42 by transformation and generated (C) Sa42-TC1 and Sa42-TC2, respectively, (D) which were then conjugated to E. coli J53 to produce the transconjugants Sa42-TC4 and Sa42-TC5 that carry fusion plasmids. (E) Plasmids in strain Sa42, Sa42-TC1, Sa42-TC2, Sa42-TC3, Sa42-TC4, and Sa42-TC5 by S1-PFGE. Conjugation of Sa42 to E. coli J53 produced Sa42-TC3. The sizes of plasmids in Sa42-TC4 and Sa42-TC5 were different from the one in Sa42-TC3. Red arrow depicts original ISEcp1 in plasmid pSa42-91k, purple arrow denotes insertion sequences ISEcp1 or IS15DI being cloned into pBackZero-T vector.