Fig. 2.

Stemness characterization of iPSC lines generated from three donors (iPSC_1–iPSC_3). (A) RT-qPCR revealed that reprogramming factor genes were successfully transfected. (B) Flow cytometry and (C) immunocytochemistry staining revealed that each iPSC line expressed embryonic stem cell markers (scale bar=100 μm). (D) Karyotyping indicated that the three iPSC lines had normal karyotypes at the eight most common karyotypic abnormality sites (chr1q, chr4p as a control, chr8q, chr10p, chr12p, chr17q, chr18q, chr20, and chrXp). Error bars indicate the range for the calculated copy number of the three replicates for each sample. (E) Detection of markers of the three germ layers verified that the iPSC lines showed pluripotency. Each gene expression level was compared before differentiation (UD) and on day 14 of differentiation (D14) using Student’s t-test. *P<0.05, ***P<0.001.

Abbreviations: iPSC, induced pluripotent stem cell; RT-qPCR, reverse transcription-quantitative PCR; UD, undifferentiated.