Fig. 5.
The hemizygosity of the S locus originated via non-homologous recombination between CFB copies. (A) Boxplot of KS values distributions for all six pairwise comparisons of the four CFB copies in the P. veris S haplotype. (B) Gene tree topology of CFB sequences from P. veris (S and s haplotypes), representing a subset of the larger CFB phylogeny of supplementary figure S28, Supplementary Material online; branch labels represent ML/parsimony bootstrap support values, inferred with RAxML and PAUP, respectively (see supplementary methods, Supplementary Material online for details). CFB underwent two duplication rounds: a tandem duplication 29.5 Ma (pink star), then a segmental duplication 2.28–4.28 Ma (green stars). (C) Schematic model for the origin of hemizygosity of the P. veris S locus via nonhomologous recombination reflecting the inferred temporal sequence of the CFB tandem and segmental duplications; the ancestral copy of CFB prior to duplication is indicated by an asterisk.