Figure 5.
The xenograft model demonstrates the efficacy of the combination of MLN4924 and celecoxib in vivo. (A–B) Nude mice bearing T24 xenograft tumors were treated with DMSO (as control), MLN4924, celecoxib, or the MLN4924/celecoxib combination for 4 weeks (n = 6 for each group, one tumor has disappeared after given the combinational drugs for 1 week). Tumor volume = longest tumor diameter × (shortest tumor diameter) 2 /2. (C–D) Tumors were fixed in 10% formalin and embedded in paraffin. Each sample was stained for Ki67 and cleaved caspase-3 with representative images shown. Positive cells were counted from three independent tumors in each group (mean ± SEM, n = 3, *0.01 < P < 0.05, **0.005 < P < 0.01, ***P < 0.005). SEM: standard error of the mean; DMSO: dimethyl sulfoxide.