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. Author manuscript; available in PMC: 2022 Feb 21.
Published in final edited form as: Methods Mol Biol. 2022;2433:105–120. doi: 10.1007/978-1-0716-1998-8_6

Figure 4: The schematic for generating vesicles encapsulating CFE reactions by cDICE method.

Figure 4:

(Top) The inner solution is made by vigorous pipetting of the CFE reaction with lipid-in-oil solution (LOS) solution. (Bottom) The cDICE contains a cylindrical chamber with a lid. Through the hole in the lid, the outer solution (OS) and LOS are sequentially introduced. The IS droplets are ejected from the pipette tip into the chamber and are then centrifuged radially towards the LOS/OS interface to form the final lipid bilayer vesicles containing CFE reactions. The top schematics were sourced from https://scidraw.io/, and the cDICE device schematic is adapted with permission from Bashirzadeh et al. (18).