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. Author manuscript; available in PMC: 2023 Feb 3.
Published in final edited form as: Eur Respir J. 2022 Feb 3;59(2):2100185. doi: 10.1183/13993003.00185-2021

Figure 1. Loss of CFTR function affects ASL pH and mucin concentration in CFTR-KO Calu3 cells.

Figure 1.

CFTR gene was knocked out in Calu3 cells via CRISPR-Cas9 to generate the CFTR-KO cell line. Wild-type (WT) and CFTR-KO Calu3 cells were analyzed via different assays. A.) Bioelectrical measurements of CFTR-mediated Isc for WT and CFTR-KO Calu3 cells showing cell response to forskolin (Fsk) (i.) and CFTR inhibitor (I-172) (ii.). n=3 per group **P<0.01, Student’s t-test B) ASL pH of WT and CFTR-KO cells measured by microprobe under CO2 and temperature-controlled conditions, n=5 per group. ****P<0.0001, Student’s t-test C) Absolute extracellular mucin concentration from WT and CFTR-KO apical washings quantified by SEC-MALS. n=4 per group. ****P<0.0001, Student’s t-test. Error bars indicate SEM.