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. Author manuscript; available in PMC: 2023 Mar 15.
Published in final edited form as: Exp Cell Res. 2022 Jan 29;412(2):113043. doi: 10.1016/j.yexcr.2022.113043

Figure 12. Inhibition of PI3K signaling induces premature spatiotermporal DNA cleavage and nuclear elimination.

Figure 12.

TUNEL assay was performed on cryosections of E12 lenses exposed for 24 hrs in organ culture to (A) DMSO, the pan-PI3K inhibitors (B) LY294002 or (C) CH5132799, or (D) the Akt-specific inhibitor MK-2206, and the sections co-labeled with the nuclear marker DAPI. Images were acquired by confocal microscopy. Boxed in regions with a single asterisk indicate the region of the central fiber zone (FC) shown at higher magnification below. Boxed in regions with a double asterisk indicate the region of the cortical fiber region (FP) shown at higher magnification below. The data shows significant premature induction of DNA cleavage and nuclear loss with the pan-PI3K inhibitors. Scale bar, 100 μm. Results are representative of 3 independent studies.