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. Author manuscript; available in PMC: 2023 Mar 15.
Published in final edited form as: Exp Cell Res. 2022 Jan 29;412(2):113043. doi: 10.1016/j.yexcr.2022.113043

Figure 4. Induction of autophagy during lens OFZ formation.

Figure 4.

Chick embryo lenses were microdissected at E11, E13, and E15 to isolate cortical fiber (FP) and central fiber (FC) zones, and were subjected to immunoblot analysis (A,B) to determine the activation state of molecules downstream of the PI3K-regulated autophagy induction pathway including (A) phosphoSer473-Akt (pAkt) and total Akt and (B) phospho-p70S6K (p-p70S6K) and total p70S6K. Quantification of the results for a minimum of 3 independent studies are shown for pAkt ser473, pAkt ser473/Akt, p-p70S6K, and p-p70S6K/p70S6K. Results are consistent with the induction of autophagy signaling during the period of lens OFZ formation. Error bars represent S.E., *P ≤ 0.05, **P ≤ 0.01, and ***P ≤ .001, t test; N.S., not significant.