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. Author manuscript; available in PMC: 2023 Mar 15.
Published in final edited form as: Exp Cell Res. 2022 Jan 29;412(2):113043. doi: 10.1016/j.yexcr.2022.113043

Figure 7. Induction of autophagy following inhibition of all PI3K downstream pathways or just the PI3K/Akt signaling axis has no effect on lens morphogenesis.

Figure 7.

Cryosections of E12 lenses were exposed in organ culture to the pan-PI3K inhibitor LY294002, the Akt-specific inhibitior MK-2206, or their vehicle DMSO for 24 hrs. (A) Lens sections immunolabeled for the lysosomal protein LAMP1 and imaged by confocal microscopy. Boxed in regions are shown on the right at higher magnification. Scale bar, 100 μm. (B) Lens sections stained with H&E and imaged by phase microscopy. Boxed regions are shown to the right at higher magnification. Results are representative of 3 independent studies.