Table 1.
Molar mass and size of co-assembled nanoreactors determined by SEC-MALS/QELS. The subscripts x and y represent molar ratios of AdhD-SP and wtSP used for assembly.
| Sample P22-[AdhD-SP]x[wtSP]y | Molar mass (MDa) | Copies of AdhD-SP | Copies of wtSP | Rrms (nm) | Rh (nm) | Rrms/ Rh |
|---|---|---|---|---|---|---|
|
| ||||||
| Empty Shell (ES) | 19.4 ± 0.4 | -------- | -------- | 25.9 ± 0.1 | 25.8 ± 0.2 | 1 ± 0.004 |
| P22-[AdhD-SP]1.00 [wtSP]0.0 | 31.10 ± 0.96 | 219 ± 6 | -------- | 20.9 ± 2.1 | 25.4 ± 1.1 | 0.82 ± 0.05 |
| P22-[AdhD-SP]0.90 [wtSP]0.10 | 30.22 ± 0.92 | 200 ± 4 | 3 ± 1 | 20.9 ± 2.3 | 25.4 ± 1.1 | 0.82 ± 0.05 |
| P22-[AdhD-SP]0.75 [wtSP]0.25 | 28.50 ± 0.74 | 154 ± 6 | 22 ± 2 | 21.6 ± 1.9 | 25.4 ± 0.8 | 0.85 ± 0.05 |
| P22-[AdhD-SP]0.50 [wtSP]0.50 | 26.94 ± 1.33 | 98 ± 8 | 74 ± 14 | 22.3 ± 1.7 | 25.4 ± 0.5 | 0.88 ± 0.05 |
| P22-[AdhD-SP]0.25 [wtSP]0.75 | 26.55 ± 1.03 | 43 ± 8 | 143 ± 11 | 22.5 ± 1.7 | 25.3 ± 0.5 | 0.89 ± 0.05 |
| P22-[AdhD-SP]0.10 [wtSP]0.90 | 26.34 ± 1.01 | 14 ± 4 | 183 ± 8 | 22.4 ± 1.7 | 25.5 ± 0.6 | 0.88 ± 0.05 |
| P22-[AdhD-SP]0.00 [wtSP]1.00 | 26.08 ± 0.83 | --------- | 194 ± 6 | 22.6 ± 1.8 | 25.6 ± 0.5 | 0.88 ± 0.05 |