Skip to main content
. Author manuscript; available in PMC: 2022 Oct 15.
Published in final edited form as: Sci Immunol. 2021 Oct 8;6(64):eabh3768. doi: 10.1126/sciimmunol.abh3768

Figure 1. Single-cell mapping of immune cell subsets in human tonsils.

Figure 1.

A) Experimental strategy for single-cell transcriptomics, surface marker expression, and chromatin accessibility of immune cells from pediatric tonsils.

B) UMAP of tonsillar immune scRNA-seq data (left; 3 donors) and scATAC-seq data (right; 7 donors).

C) Heatmap comparing gene expression, surface protein, and chromatin accessibility across immune cell types.

D) UMAP of T cell sub-populations in the tonsillar immune scRNA-seq data in B). NK = natural killer, CTL = cytotoxic lymphocyte, Treg = regulatory T cell, TfH = T follicular helper cell, Tcm = T central memory.

E) Mean expression of key marker genes for T cell sub-populations by scRNA-seq. Frequency of cells for which each gene is detected is denoted by size of the dots.

F) UMAP of B cell sub-populations in the tonsillar immune scRNA-seq data in B). MBC = memory B cell, LZ GC = light zone germinal center, DZ GC = dark zone germinal center; IFN = interferon.

G) Mean expression of key marker genes for B cell sub-populations by scRNA-seq. Frequency of cells for which each gene is detected is denoted by size of the dots.