FIGURE 6.

miRNA characterization. (a) Exosomal miR‐21, miR‐31, and U6 released from the HCT116 cell line were examined by RT‐qPCR in EVs isolated from 10 ml of CCM from cancer cell line HCT116 via UC, TEI, DTS chip, PLL‐MNPs, PEI‐MNPs, and LF‐bis‐MPA‐MNPs (LF‐MNPs). (b) Exosomal miR‐21 was examined by RT‐qPCR in EVs isolated from different volumes of CCM (1–40 ml) from cancer cell line HCT116 via UC and LF‐bis‐MPA‐MNPs. Data are presented as the mean ± standard deviation (n = 3, *p‐value ≤ 0.05)