Table 2.

Cellular effects of thyroid hormones in prostate cancer cell lines.

	Cell lines	Biological effect	References
T3
0.1 nM–1 µM for 6 days	LNCaP	Stimulates cell proliferation ([3H] thymidine incorporation).	(18)
0.1 nM–1 µM for 6 days	LNCaP DU145	Stimulates cell proliferation (MTS assay) in LNCaP but not in DU145.	(43)
10–50 ng/mL for 72 h	MDA PCa 2b	Stimulates cell proliferation ([3H] thymidine incorporation).	(8)
0.1–100 nM  0.1–10 µM for 7 days	LNCaP PC-3	Stimulates cell proliferation in LNCaP and DU145 ([3H] thymidine incorporation).	(42)
0.1 nM–1 µM for 6 days	LNCaP CA-HPV-10 PC-3 DU145	Stimulates cell proliferation (MTS assay) in LNCaP, but not in CA-HPV-10, PC-3, DU145.	(17)
10 nM for 4 or 6 days	LNCaP DU145	Reduces invasive capacity (transwell assay) stimulated by a β-adrenergic activator and the acquisition of projections like neurites (phase contrast microscopy). Treatment for 4 days had no effect in DU145.	(6)
0.1 nM–1 µM for 6 days	LNCaP	Induces senescence (β-galactosidase assay) in a dose-dependent manner.	(45)
T4
100 nM for 7 days	PC-3	Increases cell migration (transwell assay) and reduces apoptosis (flow cytometry) in anoikis-resistant cells.	(46)
100 nM for 4 or 6 days	LNCaP DU145	Increases cell invasion (transwell assay) and the acquisition of projections like neurites (phase contrast microscopy). Treatment for four days had no effect in DU145.	(6)

MTS, tetrazolium salt.