Table 1.

Kinetic constants of the fluorogenic probes catalyzed by MT1-MMP

Probes	Sequences	Km (μM)	kcat (s−1)	kcat/Km (μM−1 s−1)
P-l	(Mca)-PLGL-(Dpa)-AR-NH2	2.14 ± 0.15	0.97 × 10−3 ± 0.05 × 10−3	0.45 × 10−3 ± 0.03 × 10−3
P-3	(Mca)-PLGL-(Dpa)-AR-(PEG)3-HWKHLHNTKTFL	0.72 ± 0.03	1.20 × 10−3 ± 0.05 × 10−3	1.68 × 10−3 ± 0.74 × 10−3
P-4	(Mca)-PLGL-(Dpa)-AR-(PEG)6-HWKHLHNTKTFL	0.73 ± 0.06	3.21 × 10−3 ± 0.20 × 10−3	4.42 × 10−3 ± 0.37 × 10−3
P-5	(Mca)-PLGL-(Dpa)-AR-(PEG)12-HWKHLHNTKTFL	0.40 ± 0.03	2.44 × 10−3 ± 0.04 × 10−3	6.15 × 10−3 ± 0.44 × 10−3
P-6	(Mca)-PLGL-(Dpa)-AR-(PEG)24-HWKHLHNTKTFL	1.75 ± 0.14	1.94 × 10−3 ± 0.10 × 10−3	1.11 × 10−3 ± 0.09 × 10−3