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. 2022 Feb 14;5(5):e202101192. doi: 10.26508/lsa.202101192

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© 2022 Dominici et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S1. — Mice with QKI-depleted MuSCs exhibit reduced myogenic progenitors and defects in skeletal muscle regeneration. (A) The breeding strategy for QKI-cKO and QKI-ctrl mice is illustrated. (B) Myofibers isolated from QKI-ctrl and QKI-cKO mice were cultured for 48 h in fiber growth media and were immunostained with Pax7 (green) and pan-QKI (orange) antibodies, and counterstained with DAPI (blue). Muscle stem cells (MuSCs) are indicated with white arrows. (C) Myofibers isolated from QKI-ctrl and QKI-cKO mice and cultured for 24 h in fiber growth media were assessed for Pax7 (green) and MyoD (red) expression. MuSCs are indicated with white arrows. Scale bar represents 10 μm. (C, D) Quantification of total MuSCs from (C) (upper panel), Pax7+/MyoD− and Pax7+/MyoD+ (lower panels) (n = 3 biological replicates, minimum 100 cells per group was quantified, ns, not significant).