Figure S3.

Components of MuSC polarity establishment undergo AS in QKI-deficient MuSCs. (A) Sashimi plots generated using rMATs software of asymmetric cell division factors. Dmd, Dystrophin; Mark2, Microtubule affinity regulating kinase; Itga7, Integrin alpha-7. WT: QKI-ctrl (red track); QKIcko: QKI-cKO (yellow track). (B) RT–PCR validation of SE events of Dmd, Mark2, and Numb in primary muscle stem cells (MuSCs) treated with siQKI or siLuc control and cultured in differentiation media for 1 d (1DM), or 2 d (2DM). (C) Pathway enrichment analysis carried out using Enrichr software of alternative last exon events in QKI-cKO versus QKI-ctrl MuSCs. (D) RT–PCR validation of alternative splicing events of select sarcomere components in proliferating MuSCs transfected with siQKI or siLuc. PCR products were separated on acrylamide gels and stained with ethidium bromide. The migration of DNA markers in base pairs (bp) is shown on the left, and alternative spliced exon and its inclusion/exclusion is depicted on the right. Capzb, Capping actin protein of muscle Z-line subunit beta; Tpm1, Tropomyosin 1; Tnnt3, Troponin T3; Neb, Nebulin.