Figure 8. Effect of EZH1 or EZH2 gain- or loss-of-function on smooth muscle cell proliferation and migration.

(A, B, C, D) Loss of function. Silencing efficiency is indicated by Western blots (A). For proliferation (B) and migration (C, D) assays, cells were harvested at 72 h or imaged at 24 h after PDGF-BB stimulation, respectively. Scr, scrambled. NS, not significant. (E, F, G, H) Gain of function. EZH1 or EZH2 overexpression is shown in Western blots (E). * marks the recombinant protein in fusion with GFP; the lower band is endogenous protein. For proliferation (F) and migration (G, H) assays, cells were harvested at 72 h or imaged at 24 h after PDGF-BB stimulation, respectively. Quantification: Mean ± SEM; n = 3 independent experiments. Statistics: one-way ANOVA with Bonferroni test, *P < 0.05.

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