Figure S8. pan-EZH inhibitor mitigates smooth muscle cell proliferation and migration.

MOVAS cells were cultured, starved for 6 h, pretreated with vehicle (DMSO) or the pan-EZH1/2 inhibitor UNC1999 for 2 h, and then stimulated with PDGF-BB (final 20 ng/ml). (A, B) For proliferation (A) and migration (B, 5 μM UNC1999) assays, cells were harvested at 72 h or imaged at 24 h after PDGF-BB stimulation, respectively. For the scratch assay, the calcein dye was added at the end of 24 h PDGF treatment to illuminate the cells. The yellow lines on the 0 h picture demarcate the original scratched cell-free gap.