KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-actin (clone C4) | EMD Millipore | Cat# MAB1501 |
| Mouse anti-β-actin | Abcam | Cat# ab8226 |
| Mouse anti-smooth muscle α-actin (ACTA2) | Abcam | Cat# ab220179 |
| Mouse anti-FLAG | Sigma-Aldrich | Cat# F3165 |
| Rabbit anti-Histone H3Ac | Active Motif | Cat# 61637 |
| Rabbit anti-Histone H4 | Abcam | Cat# ab7311 |
| Rabbit anti-Caspase-3 | Abcam | Cat# ab32042 |
| Rabbit anti-PRMT1 | Abcam | Cat# ab190892 |
| Rabbit anti-PRMT2 | Abcam | Cat# ab154154 |
| Rabbit anti-PRMT3 | Abcam | Cat# ab191562 |
| Rabbit anti-PRMT4 | Abcam | Cat# ab128851 |
| Rabbit anti-PRMT5 | Abcam | Cat# ab109451 |
| Rabbit anti-PRMT6 | Abcam | Cat# ab72205 |
| Rabbit anti-PRMT7 | Abcam | Cat# ab181214 |
| Rabbit anti-actin R256me1 | This study | N/A |
| Rabbit anti-actin K61me1 | This study | N/A |
| Rabbit anti-actin K61Ac | This study | N/A |
| Rabbit anti-actin K326me1 | This study | N/A |
| Rabbit anti-actin K326Ac | This Study | N/A |
| Biological Samples | ||
| Fetal Bovine Serum | GIBCO | Cat# 26-140-095 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Rabbit muscle actin | Cytoskeleton Inc. | Cat# AKL99-A |
| Recombinant PRMT5 | Sigma-Aldrich | Cat# #SRP0146-20UG |
| ReadyPrep 2D-Starter Kit Rehydration/Sample Buffer | BIO-RAD | Cat# 1632106 |
| DMEM | Corning | Cat# 10013-CM |
| Pen-Strep-Ampho | Biological Industries | Cat# #03033-1B |
| Lipofectamine RNAiMAX Transfection Reagent | Thermo Scientific | Cat# #13778030 |
| RIPA Buffer | G Biosciences | Cat# 786-490 |
| S-Adenosyl Methionine | Sigma-Aldrich | Cat# A9384 |
| CMP5, PRMT5 inhibitor | Sigma-Aldrich | Cat# 5343560001 |
| FLAG peptide | Sigma-Aldrich | Cat# F3290-25MG |
| Critical Commercial Assays | ||
| Nuclear/Cytosolic Fractionation Kit | Cell Biolabs, Inc. | Cat# ARK-171 |
| TruSeq Stranded mRNA kit | Illumina | Cat# RS-122-2101t |
| Deposited Data | ||
| Raw data | This paper | GEO: GSE156817 |
| Experimental Models: Cell Lines | ||
| HEK293 | Sigma-Aldrich | Cat# 85120602-1VL |
| NIH 3T3 | Sigma-Aldrich | Cat# 93061524-1VL |
| Software and Algorithms | ||
| ClustalW2 | http://www.clustal.org/clustal2/ | Larkin et al., 2007 |
| MEGA6 | https://www.megasoftware.net | Tamura et al., 2013 |
| Gnuplot4.6 | http://www.gnuplot.info | Williams and Kelley, 2012 |
| PyMOL | Schrodinger, Inc. | The PyMOL Molecular Graphics System |
| R | https://www.r-project.org | Albert and Redon, 1998 |
| VMD | http://www.clustal.org/clustal2/ | Humphrey et al., 1996 |
| AMBER14 | http://ambermd.org | Case et al., 2014 |
| CPPTRAJ | http://ambermd.org | Roe and Cheatham, 2013 |
| NMWiz | http://prody.csb.pitt.edu | Bakan et al., 2011 |
| Prody | http://prody.csb.pitt.edu | Bakan et al., 2011 |
| DESeq2 Bioconductor package | https://github.com/mikelove/DESeq2 | Anders and Huber, 2010; Anders et al., 2013 |
| GSEA | https://www.broadinstitute.org/gsea | Mootha et al., 2003; Subramanian et al., 2005 |
| GraphPad Prism 7 | GraphPad Software | N/A |