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. 2022 Jan 13;42(3):326–342. doi: 10.1161/ATVBAHA.121.317093

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© 2022 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 6. — Endothelial-to-mesenchymal transition (EndoMT) and vascular calcification were increased in Tie2 (Tek receptor tyrosine kinase)-cre–mediated ECs-specific microRNA-126 (miR-126) knockout (miR-126-ecKO) embryos. A, Five subclusters were obtained by further clustering of EndoMT (cluster 10 in Figure 6A). Colors denote different subclusters or mice line. B, Fractions of EndoMT from miR-126-ecKO or miR-126^fl/fl in each subcluster. C, Gene Ontology biological function analysis of differentially expressed genes in cluster 3. D, Feather plot of representative genes in chondrogenesis and endothelial-to-mesenchymal which were enriched in cluster 3. E, Double immunofluorescent staining of COL1A1 (alpha-1 type I collagen) and PECAM1 (platelet endothelial cell adhesion molecule-1) proteins in embryonic hepatic tissue to identify the EndMT (Endothelial-to-Mesenchymal Transition). F, Alizarin Red S Staining to identify the calcium in the adult’s brain and lung slides. Scale bar=100 µm. DAPI indicates 4',6-diamidino-2-phenylindole.