Figure 5.

Immunostaining of a cardiac valve vegetation in a model of inflammation-induced IE. IE vegetation was induced upon infection of mice with S. aureus and treatment with local histamine infusion via catheter instillation. A, Brightfield image of a Gram stain with bacteria seen in purple. B–D, Fluorescence images of the cardiac valve vegetation showing: neutrophil-specific marker Ly6G (red) and S. aureus (green) (B); platelet CD41 (red) and fibrinogen (green) (C); VWF (Von Willebrand Factor) in green (D). DNA is identified by Hoechst 33342 staining, depicted in blue. Images were acquired using a Zeiss Axioscan Z1 digital slide scanner or Zeiss Axiovert inverted microscope at the VIB-KU Leuven LiMoNe Bio Imaging Core and the KU Leuven Department of Cardiovascular Sciences Microscopy Core, respectively.