Table 2.
Proteins identified by LC–MSE
| Gel band | Protein (organism) | Mass (Da)a | UniProtKB AC | Score | Coverage (%) | Significant sequencesb |
|---|---|---|---|---|---|---|
| V | Vimentin (Homo sapiens) | 53 676 | P08670 | 746 | 54 | 25 |
| S2 | Streptavidin (Streptomyces avidinii) | 18 822 | P22629 | 333 | 37 | 4 |
| S1 | Streptavidin (Streptomyces avidinii) | 18 822 | P22629 | 432 | 37 | 4 |
aMolecular monoisotopic mass of the UniProt sequence after cysteine carbamidomethylation.
bNumber of significant peptides sequenced by LC–MSE. The significant peptides identified for each protein with their expectation values are listed in Supplementary Material-Excel file.