Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 20;50(3):1734–1752. doi: 10.1093/nar/gkac022

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — tRNAs are processed by Dicer into tsRNA. (A) Box plots showing the log2 fold change of various tsRNA types, miRNA and snoRNA upon Dicer knockdown. (B) Box plots showing Dicer dependent tsRNA derived from various tRNA isotypes (C). Bar charts representing the length distribution of various tsRNA types based on sequence length, in nucleotides (nt). (D). Images of northern blot showing signals of tRNA and corresponding tRFs for Pro, and Ala in wild type HEK293T and an inducible Dicer knockdown (shDicer) cell line. (E) Stem loop qRT-PCR showing levels of selected tsRNAs and miRNAs fragments isolated from wt and Dicer KD cells. The values are derived from three biological repeats and each in the bar chart corresponds to average value of two technical repeats. (F) Northern blot images showing signals of tRNA^Pro and its corresponding tsRNA (tRF-3^Pro), and tRNA^Ala at different time points after the addition of FLAG-tagged Dicer or TAP-tagged Dicer.