Table 2.

Properties of fully deprotected PS dinucleotide diastereomers

		Rp a				Sp b
Dimer	HRMS calc.	HRMS found	31P NMRe	SVPDf	PDIIg	HRMS found	31P NMRe	SVPDf	PDIIg
usu	617.09c	617.09c	56.74	deg.	-	617.09c	55.55	no deg.	-
usUf	605.07c	605.07c	56.78	deg.	-	605.07c	55.68	no deg.	-
Ufsu	605.07c	605.07c	56.82	deg.	no deg.	605.07c	55.97	no deg.	deg.
asa	641.17d	641.17d	57.51	deg.	no deg.	641.17d	56.27	no deg.	deg.
Afsa	629.15d	629.15d	57.21	deg.	no deg.	629.15d	55.40	no deg.	deg.
asUf	606.12d	606.12d	57.83	deg.	no deg.	606.12d	56.63	no deg.	deg.
gsa	657.16d	657.16d	56.07	no deg.	deg.	657.16d	57.53	deg.	no deg.

^aThe Rp diastereomer was the top spot on TLC for all dinucleotides except for gsa.

^bThe Sp diastereomer was the lower spot on TLC for all dinucleotides except gsa.

^cHRMS, high-resolution mass spectrometry: (M+Na).

^dHRMS: (M+H).

^eChemical shift (ppm) in ³¹P NMR spectrum of fully deprotected dinucleotide in D₂O.

^fSVPD degradation at 48 h time point.

^gPDII degradation at 48 h time point. deg. indicates that there was degradation; no deg. indicates no degradation.

Lowercase u, a, and g indicate 2′-OMe nucleosides, lowercase s indicates PS linkage, and Uf and Af indicate 2′-F U and A nucleosides, respectively.