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. 2022 Jan 20;50(3):1661–1672. doi: 10.1093/nar/gkab1299

Figure 4.

Figure 4.

Cas1 and Cas2 are sufficient to determine the observed S. epidermidis type III-A acquisition pattern. (A) Schematic representation of the two-plasmid system used in the inducible adaptation assay for S. epidermidis type III-A dcsm6 CRISPR-Cas system. (B) Agarose gel electrophoresis DR-PCR products at different time points post induction of CRISPR adaptation in the presence of pCRISPR(dcsm6). (C) Abundance (RPM) of spacers acquired 3 h post induction of the S. epidermidis type III-A acquisition machinery in host cells harboring pCRISPR(dcsm6), mapped to the bacterial chromosome (10 kb bins). (D) Schematic representation of the two-plasmid system used in the inducible adaptation assay for S. epidermidis type III-A pDR (i.e. Δcas10-6 CRISPR-Cas system). (E) Agarose gel electrophoresis DR-PCR products at different time points post induction of CRISPR adaptation in the presence of pDR. (F) Abundance (RPM) of spacer acquired 3 h post induction of the S. epidermidis type III-A acquisition machinery in host cells harboring pDR, mapped to the bacterial chromosome (10 kb bins).