REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Anti-NP1 | Genscript | U864YFA140-4/CB2093 |
anti-rabbit IgG | Vector Laboratories | Cat #MP-6401 |
Goat anti-hamster IgG HRP | KPL | Cat #PA1-29626 |
Bacterial and virus strains | ||
SARS-CoV-2 isolate SARS-CoV-2/human/USA/WA-CDC-WA1/2020 (WA1) | CDC Atlanta | Genbank Accession MN985325.1 |
SARS-CoV-2 isolate nCoV-hCoV-19/USA/MD-HP01542/2021 (B.1.351; SA; beta) | Andy Pekosz (Johns Hopkins) | GISAID # EPI_ISL_890360 |
SARS-CoV-2_2hCOV_19_England_204820464_ 2020 isolate (B.1.1.7; UK; alpha) | BEI Resources | GISAID # EPI_ISL_683466 |
Critical commercial assays | ||
Rotor-Gene probe kit | Qiagen | Cat #204374 |
QiaAmp Viral RNA kit | Qiagen | Cat #52906 |
RNeasy kit | Qiagen | Cat #74004 |
ChromoMap DAB kit | Roche Tissue Diagnostics | Cat#760-159 |
Experimental models: Cell lines | ||
Vero E6 | Ralph Baric, UNC | N/A |
Experimental models: Organisms/strains | ||
Syrian hamster HsdHan:AURA | Envigo | N/A |
Oligonucleotides | ||
Subgenomic E primer forward 5’-ACAGGTACGTT AATAGTTAATAGCGT |
IDT | N/A |
Subgenomic E primer reverse 5’-ATATTGCAGCA GTACGCACACA |
IDT | N/A |
Subgenomic E probe 5’-FAM-ACACTAGCCATCC TTACTGCGCTTCG-BBQ |
IDT | N/A |
Software and algorithms | ||
Prism 9 | Graphpad | https://www.graphpad.com/scientific-software/prism/ |