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. 2022 Feb 22;38(11):110515. doi: 10.1016/j.celrep.2022.110515
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Anti-NP1 Genscript U864YFA140-4/CB2093
anti-rabbit IgG Vector Laboratories Cat #MP-6401
Goat anti-hamster IgG HRP KPL Cat #PA1-29626

Bacterial and virus strains

SARS-CoV-2 isolate SARS-CoV-2/human/USA/WA-CDC-WA1/2020 (WA1) CDC Atlanta Genbank Accession MN985325.1
SARS-CoV-2 isolate nCoV-hCoV-19/USA/MD-HP01542/2021 (B.1.351; SA; beta) Andy Pekosz (Johns Hopkins) GISAID # EPI_ISL_890360
SARS-CoV-2_2hCOV_19_England_204820464_ 2020 isolate (B.1.1.7; UK; alpha) BEI Resources GISAID # EPI_ISL_683466

Critical commercial assays

Rotor-Gene probe kit Qiagen Cat #204374
QiaAmp Viral RNA kit Qiagen Cat #52906
RNeasy kit Qiagen Cat #74004
ChromoMap DAB kit Roche Tissue Diagnostics Cat#760-159

Experimental models: Cell lines

Vero E6 Ralph Baric, UNC N/A

Experimental models: Organisms/strains

Syrian hamster HsdHan:AURA Envigo N/A

Oligonucleotides

Subgenomic E primer forward 5’-ACAGGTACGTT
AATAGTTAATAGCGT
IDT N/A
Subgenomic E primer reverse 5’-ATATTGCAGCA
GTACGCACACA
IDT N/A
Subgenomic E probe 5’-FAM-ACACTAGCCATCC
TTACTGCGCTTCG-BBQ
IDT N/A

Software and algorithms

Prism 9 Graphpad https://www.graphpad.com/scientific-software/prism/