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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: J Invest Dermatol. 2021 Sep 10;142(3 Pt A):692–704.e14. doi: 10.1016/j.jid.2021.08.428

Figure 4. Blocking IL-10 signaling dampens IL-10 and enhances TLR signaling and proinflammatory cytokines production in diabetic wound.

Figure 4.

(a-e) db/db wounds were treated topically with α-IgG or α-IL10 antibodies (10μg/wound), immediately after wounding. Wound tissues were analyzed at indicated timepoints for IL-10 by ELISA (a), for the expression of indicated genes by Western blotting (b), by RT-PCR (c), by IHC (d), and the corresponding data for (d) are plotted as the mean ± SEM in (e). (N=5 mice/group for all experiments. For (d-e) ≥9 random fields/wound/mouse, red scale bars=50μm. Data were then normalized per wound area and plotted as mean ± SEM, *p<0.05, ** p<0.01, ***p<0.001, Statistical analyses between groups were performed by One-way ANOVA, and pair-wise comparisons within groups were performed or by unpaired Student’s t-test).