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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: J Invest Dermatol. 2021 Sep 10;142(3 Pt A):692–704.e14. doi: 10.1016/j.jid.2021.08.428

Figure 5. Blocking IL-10 signaling enhances inflammatory responses during the acute phase of wound healing.

Figure 5.

db/db wounds were treated with α-IgG or α-IL10 (10μg/wound) after wounding and assessed at indicated timepoints for indicated proinflammatory cytokines (a-c), for leukocytes by H&E staining (d-e), and for M1 and M2 macrophages, by IF microscopy using F4/80/iNOS or F4/80/MMR/CD206 respectively (f-g). The corresponding data of (d, f, & g) are plotted as mean ± SEM (Red scale bars=50μm, White scale bar= 50 μm) in (e, h, & i) respectively. M1/M2 macrophage ratios are shown in (j). (N=5 mice/group; ≥9 random fields/wound/mouse. The number of leukocytes and macrophages were normalized per wound area. *p<0.05, ** p<0.01, ***p<0.001. Statistical analyses between groups were performed by One-way ANOVA, and pair-wise comparisons within groups were performed or by unpaired Student t-test).