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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: J Invest Dermatol. 2021 Sep 10;142(3 Pt A):692–704.e14. doi: 10.1016/j.jid.2021.08.428

RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
NF-κB p65 (D14E12) XP® Rabbit mAb Cell Signaling Technology Cat. No. 8242
IL-1β (3A6) Mouse mAb Cell Signaling Technology Cat. No. 12242
F4/80 (D2S9R) XP® Rabbit mAb Cell Signaling Technology Cat. No. 70076
Mouse (G3A1) mAb IgG1 Isotype Control Cell Signaling Technology Cat. No. 5415
GAPDH Antibody Rabbit Polyclonal Proteintech Cat. No. 1094-I-AP
MCP-1 Antibody (Mouse Specific) Cell Signaling Technology Cat. No. 2029
TLR1 Rat anti-Mouse, Clone: 285923 R &D systems Cat. No. MAB1475
TLR2 Goat anti-Mouse, Polyclonal R &D systems Cat. No. AF1530
TLR4 Antibody (25) Santa Cruz Biotechnology Cat. No. 293072
IL-10 Antibody (E-10) Santa Cruz Biotechnology Cat. No. 8438
TLR2 Antibody (TL2.1) Santa Cruz Biotechnology Cat. No. 21759
TNF alpha Antibody Santa Cruz Biotechnology Cat. No. 52746
Anti-MyD88 antibody Abcam Cat. No. ab135693
Anti-IL-10 antibody [JES5-2A5] Abcam Cat. No. ab33471
InVivoMAb anti-mouse IL-10R (CD210), clone: 1B1.3A InVivoMab Antibodies Cat. No. BE0050
Recombinant Anti-G-CSF antibody [EPR3203(N)(B)] Abcam Cat. No. ab181053
TRAF6 Polyclonal Antibody Invitrogen Cat. No. 38-0900
GM-CSF Monoclonal Antibody (22E9) Invitrogen Cat. No. MM500C
IL-10 Monoclonal Antibody (JES3-9D7) Invitrogen Cat. No. AHC0102
Mouse (MOPC-21) mAb IgG1 Isotype Control Cell Signaling Technology Cat. No. 4097
InVivoMAb anti-mouse IL-10, Clone: JES5-2A5 InVivoMab Antibodies Cat. No. BE0049
anti-horseradish peroxidase, Clone: HRPN InVivoMab Antibodies Cat. No. BE0088
F4/80 (D2S9R) XP® Rabbit mAb Cell Signaling Technology Cat. No. 70076
MMR/CD206/Mannose Receptor Antibody Novus Biologicals Cat no. NBP1-90020
iNOS Antibody Novus Biologicals Cat no. NBP2-22119
F4/80 Antibody (C-7) Santa Cruz Biotechnology Cat no. 377009
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 Thermo Fisher Cat no. A11-012
Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (H+L) Jackson Immuno Research Cat no. 115-545-062
Anti-α-SMA antibody Abcam Cat. No. ab5694
Anti-vimentin antibody Abcam Cat. No. ab92547
Reagents and kits
Hematoxylin Thermo Fisher Cat. No. 7111L
Eosin Y Thermo Fisher Cat. No. 7211L
Bluing Reagent Thermo Fisher Cat. No. 7301L
Masson’s Trichrome stain Abcam Cat. No. ab150686
EasySep Human Monocytes Enrichment Kit STEMCELL Technologies Cat. No. 19359
EasySep Mouse monocytes Enrichment Kit STEMCELL Technologies Cat. No. 19861
EasySep Buffer STEMCELL Technologies Cat. No. 20144
SYBR™ Green PCR Master Mix Thermo Fisher Cat. No. 4309155
SuperScript™ III First-Strand Synthesis System Thermo Fisher Cat. No. 18080051
Elastic connective tissue stain Abcam Cat. No. ab150667
Critical Commercial Assays
CCL2 ELISA kit Thermo Fisher Cat. No. 88-7391-22
IL-1β ELISA kit Thermo Fisher Cat. No. 88-7013-88
TNF-α ELISA kit Thermo Fisher Cat. No. 88-7324-88
EZCell™ Glucose Uptake Assay kit BioVision Cat. No. K924
Experimental Models: Organisms
Mouse: C57BL/6J Jackson laboratories 000664
Mouse: C57BLKS-m leprdb(db/db) Jackson laboratories 000642
Oligonucleotides
Human:
Tlr2 Forward: GAAGAGTGAGTGGTGCAAGTAT, Reverse: AATGGGCTCCAGAAGAATGAG; Integrated DNA Technologies N/A
Tlr4 Forward: TTTCAGCTCTGCCTTCACTAC, Reverse: GACACCACAACAATCACCTTTC Integrated DNA Technologies N/A
MYD88 Forward: CTGTGTCTGGTCTATTGCTAGTG, Reverse: TTCCTTGCTCTGCAGGTAATC Integrated DNA Technologies N/A
IL1 Forward: CAAAGGCGGCCAGGATATAA, Reverse: CTAGGGATTGAGTCCACATTCAG Integrated DNA Technologies N/A
TNF Forward: GCAGGTCTACTTTGGGATCATT, Reverse: AGAAGAGGTTGAGGGTGTC Integrated DNA Technologies N/A
RelA Forward: CTGTCCTTTCTCATCCCATCTT, Reverse: TCCTCTTTCTGCACCTTGTC Integrated DNA Technologies N/A
RelB Forward: CTGCGGATTTGCCGAATTAAC, Reverse: ACACCACTGATATGTCCTCTTTC Integrated DNA Technologies N/A
Ccl2 Forward: TCATAGCAGCCACCTTCATTC, Reverse: CTCTGCACTGAGATCTTCCTATTG Integrated DNA Technologies N/A
Ccl3 Forward: GGCAGATTCCACAGAATTTCATAG, Reverse: TCGCTTGGTTAGGAAGATGAC Integrated DNA Technologies N/A
IL10 Forward: TCCTTGCTGGAGGACTTTAAGGGT, Reverse: TGTCTGGGTCTTGGTTCTCAGCTT Integrated DNA Technologies N/A
18s Forward: CACGGACAGGATTGACAGATT, Reverse: GCCAGAGTCTCGTTCGTTATC Integrated DNA Technologies N/A
GAPDH Forward: GGTGTGAACCATGAGAAGTATGA, Reverse: GAGTCCTTCCACGATACCAAAG Integrated DNA Technologies N/A
Mice:
granulocyte (Csf3) Forward: TGTTCCCAAACTGGGTTCTT, Reverse: TGGCTGCCACTGTTTCTT Integrated DNA Technologies N/A
GM-CSF Forward: AGCTCTGAATCCAGCTTCTC, Reverse: CCACATCTCTTGGTCCCTTTA Integrated DNA Technologies N/A
Ccl2 Forward: CTCACCTGCTGCTACTCATTC, Reverse: ACTACAGCTTCTTTGGGACAC Integrated DNA Technologies N/A
Ccl3 Forward: TCACTGACCTGGAACTGAATG, Reverse: CAGCTTATAGGAGATGGAGCTATG Integrated DNA Technologies N/A
Rela Forward: CCGACTTGTTTGGGTGATCT, Reverse: TCCGTCTCCAGGAGGTTAAT Integrated DNA Technologies N/A
Relb Forward: TGCCGAATCAACAAGGAGAG, Reverse: TGCTGAACACCACGGATATG Integrated DNA technologies N/A
Tlr2 Forward: CACTATCCGGAGGTTGCATATC, Reverse: GGAAGACCTTGCTGTTCTCTAC Integrated DNA Technologies N/A
Tlr4 Forward: GAGCAAACAGCAGAGGAAGA, Reverse: CCAGGTGAGCTGTAGCATTTA Integrated DNA Technologies N/A
Myd88 Forward: AGCAACTAGGACTGCCTTTC, Reverse: GAACTCTTCCACTCAGCTATCC Integrated DNA Technologies N/A
IL-1 Forward: GCACTACAGGCTCCGAGATGAAC; Reverse: TTGTCGTTGCTTGGTTCTCCTTGT Integrated DNA Technologies N/A
Tnf Forward: TTGTCTACTCCCAGGTTCTCT, Reverse: GAGGTTGACTTTCTCCTGGTATG Integrated DNA Technologies N/A
GAPDH Forward: TTGGGTTGTACATCCAAGCA, Reverse: CAAGAAACAGGGGAGCTGAG Integrated DNA Technologies N/A
Cell lines
Mouse Primary Epidermal Keratinocytes Cell Biologics Cat No: C57-6066K
Mouse Primary Dermal Lymphatic Endothelial Cells Cell Biologics Cat No: C57-6064L
Software and Algorithms
GraphPad Prism GraphPad https://graphpad.com/scientific-software/prism/