Antibodies |
NF-κB p65 (D14E12) XP® Rabbit mAb |
Cell Signaling Technology |
Cat. No. 8242 |
IL-1β (3A6) Mouse mAb |
Cell Signaling Technology |
Cat. No. 12242 |
F4/80 (D2S9R) XP® Rabbit mAb |
Cell Signaling Technology |
Cat. No. 70076 |
Mouse (G3A1) mAb IgG1 Isotype Control |
Cell Signaling Technology |
Cat. No. 5415 |
GAPDH Antibody Rabbit Polyclonal |
Proteintech |
Cat. No. 1094-I-AP |
MCP-1 Antibody (Mouse Specific) |
Cell Signaling Technology |
Cat. No. 2029 |
TLR1 Rat anti-Mouse, Clone: 285923 |
R &D systems |
Cat. No. MAB1475 |
TLR2 Goat anti-Mouse, Polyclonal |
R &D systems |
Cat. No. AF1530 |
TLR4 Antibody (25) |
Santa Cruz Biotechnology |
Cat. No. 293072 |
IL-10 Antibody (E-10) |
Santa Cruz Biotechnology |
Cat. No. 8438 |
TLR2 Antibody (TL2.1) |
Santa Cruz Biotechnology |
Cat. No. 21759 |
TNF alpha Antibody |
Santa Cruz Biotechnology |
Cat. No. 52746 |
Anti-MyD88 antibody |
Abcam |
Cat. No. ab135693 |
Anti-IL-10 antibody [JES5-2A5] |
Abcam |
Cat. No. ab33471 |
InVivoMAb anti-mouse IL-10R (CD210), clone: 1B1.3A |
InVivoMab Antibodies |
Cat. No. BE0050 |
Recombinant Anti-G-CSF antibody [EPR3203(N)(B)] |
Abcam |
Cat. No. ab181053 |
TRAF6 Polyclonal Antibody |
Invitrogen |
Cat. No. 38-0900 |
GM-CSF Monoclonal Antibody (22E9) |
Invitrogen |
Cat. No. MM500C |
IL-10 Monoclonal Antibody (JES3-9D7) |
Invitrogen |
Cat. No. AHC0102 |
Mouse (MOPC-21) mAb IgG1 Isotype Control |
Cell Signaling Technology |
Cat. No. 4097 |
InVivoMAb anti-mouse IL-10, Clone: JES5-2A5 |
InVivoMab Antibodies |
Cat. No. BE0049 |
anti-horseradish peroxidase, Clone: HRPN |
InVivoMab Antibodies |
Cat. No. BE0088 |
F4/80 (D2S9R) XP® Rabbit mAb |
Cell Signaling Technology |
Cat. No. 70076 |
MMR/CD206/Mannose Receptor Antibody |
Novus Biologicals |
Cat no. NBP1-90020 |
iNOS Antibody |
Novus Biologicals |
Cat no. NBP2-22119 |
F4/80 Antibody (C-7) |
Santa Cruz Biotechnology |
Cat no. 377009 |
Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 |
Thermo Fisher |
Cat no. A11-012 |
Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (H+L) |
Jackson Immuno Research |
Cat no. 115-545-062 |
Anti-α-SMA antibody |
Abcam |
Cat. No. ab5694 |
Anti-vimentin antibody |
Abcam |
Cat. No. ab92547 |
Reagents and kits
|
Hematoxylin |
Thermo Fisher |
Cat. No. 7111L |
Eosin Y |
Thermo Fisher |
Cat. No. 7211L |
Bluing Reagent |
Thermo Fisher |
Cat. No. 7301L |
Masson’s Trichrome stain |
Abcam |
Cat. No. ab150686 |
EasySep Human Monocytes Enrichment Kit |
STEMCELL Technologies |
Cat. No. 19359 |
EasySep Mouse monocytes Enrichment Kit |
STEMCELL Technologies |
Cat. No. 19861 |
EasySep Buffer |
STEMCELL Technologies |
Cat. No. 20144 |
SYBR™ Green PCR Master Mix |
Thermo Fisher |
Cat. No. 4309155 |
SuperScript™ III First-Strand Synthesis System |
Thermo Fisher |
Cat. No. 18080051 |
Elastic connective tissue stain |
Abcam |
Cat. No. ab150667 |
Critical Commercial Assays
|
CCL2 ELISA kit |
Thermo Fisher |
Cat. No. 88-7391-22 |
IL-1β ELISA kit |
Thermo Fisher |
Cat. No. 88-7013-88 |
TNF-α ELISA kit |
Thermo Fisher |
Cat. No. 88-7324-88 |
EZCell™ Glucose Uptake Assay kit |
BioVision |
Cat. No. K924 |
Experimental Models: Organisms
|
Mouse: C57BL/6J |
Jackson laboratories |
000664 |
Mouse: C57BLKS-m leprdb(db/db) |
Jackson laboratories |
000642 |
Oligonucleotides
|
Human:
|
Tlr2 Forward: GAAGAGTGAGTGGTGCAAGTAT, Reverse: AATGGGCTCCAGAAGAATGAG; |
Integrated DNA Technologies |
N/A |
Tlr4 Forward: TTTCAGCTCTGCCTTCACTAC, Reverse: GACACCACAACAATCACCTTTC |
Integrated DNA Technologies |
N/A |
MYD88 Forward: CTGTGTCTGGTCTATTGCTAGTG, Reverse: TTCCTTGCTCTGCAGGTAATC |
Integrated DNA Technologies |
N/A |
IL1 Forward: CAAAGGCGGCCAGGATATAA, Reverse: CTAGGGATTGAGTCCACATTCAG |
Integrated DNA Technologies |
N/A |
TNF Forward: GCAGGTCTACTTTGGGATCATT, Reverse: AGAAGAGGTTGAGGGTGTC |
Integrated DNA Technologies |
N/A |
RelA Forward: CTGTCCTTTCTCATCCCATCTT, Reverse: TCCTCTTTCTGCACCTTGTC |
Integrated DNA Technologies |
N/A |
RelB Forward: CTGCGGATTTGCCGAATTAAC, Reverse: ACACCACTGATATGTCCTCTTTC |
Integrated DNA Technologies |
N/A |
Ccl2 Forward: TCATAGCAGCCACCTTCATTC, Reverse: CTCTGCACTGAGATCTTCCTATTG |
Integrated DNA Technologies |
N/A |
Ccl3 Forward: GGCAGATTCCACAGAATTTCATAG, Reverse: TCGCTTGGTTAGGAAGATGAC |
Integrated DNA Technologies |
N/A |
IL10 Forward: TCCTTGCTGGAGGACTTTAAGGGT, Reverse: TGTCTGGGTCTTGGTTCTCAGCTT |
Integrated DNA Technologies |
N/A |
18s Forward: CACGGACAGGATTGACAGATT, Reverse: GCCAGAGTCTCGTTCGTTATC |
Integrated DNA Technologies |
N/A |
GAPDH Forward: GGTGTGAACCATGAGAAGTATGA, Reverse: GAGTCCTTCCACGATACCAAAG |
Integrated DNA Technologies |
N/A |
Mice:
|
granulocyte (Csf3) Forward: TGTTCCCAAACTGGGTTCTT, Reverse: TGGCTGCCACTGTTTCTT |
Integrated DNA Technologies |
N/A |
GM-CSF Forward: AGCTCTGAATCCAGCTTCTC, Reverse: CCACATCTCTTGGTCCCTTTA |
Integrated DNA Technologies |
N/A |
Ccl2 Forward: CTCACCTGCTGCTACTCATTC, Reverse: ACTACAGCTTCTTTGGGACAC |
Integrated DNA Technologies |
N/A |
Ccl3 Forward: TCACTGACCTGGAACTGAATG, Reverse: CAGCTTATAGGAGATGGAGCTATG |
Integrated DNA Technologies |
N/A |
Rela Forward: CCGACTTGTTTGGGTGATCT, Reverse: TCCGTCTCCAGGAGGTTAAT |
Integrated DNA Technologies |
N/A |
Relb Forward: TGCCGAATCAACAAGGAGAG, Reverse: TGCTGAACACCACGGATATG |
Integrated DNA technologies |
N/A |
Tlr2 Forward: CACTATCCGGAGGTTGCATATC, Reverse: GGAAGACCTTGCTGTTCTCTAC |
Integrated DNA Technologies |
N/A |
Tlr4 Forward: GAGCAAACAGCAGAGGAAGA, Reverse: CCAGGTGAGCTGTAGCATTTA |
Integrated DNA Technologies |
N/A |
Myd88 Forward: AGCAACTAGGACTGCCTTTC, Reverse: GAACTCTTCCACTCAGCTATCC |
Integrated DNA Technologies |
N/A |
IL-1 Forward: GCACTACAGGCTCCGAGATGAAC; Reverse: TTGTCGTTGCTTGGTTCTCCTTGT |
Integrated DNA Technologies |
N/A |
Tnf Forward: TTGTCTACTCCCAGGTTCTCT, Reverse: GAGGTTGACTTTCTCCTGGTATG |
Integrated DNA Technologies |
N/A |
GAPDH Forward: TTGGGTTGTACATCCAAGCA, Reverse: CAAGAAACAGGGGAGCTGAG |
Integrated DNA Technologies |
N/A |
Cell lines
|
Mouse Primary Epidermal Keratinocytes |
Cell Biologics |
Cat No: C57-6066K |
Mouse Primary Dermal Lymphatic Endothelial Cells |
Cell Biologics |
Cat No: C57-6064L |
Software and Algorithms
|
GraphPad Prism |
GraphPad |
https://graphpad.com/scientific-software/prism/
|