RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| NF-κB p65 (D14E12) XP® Rabbit mAb | Cell Signaling Technology | Cat. No. 8242 |
| IL-1β (3A6) Mouse mAb | Cell Signaling Technology | Cat. No. 12242 |
| F4/80 (D2S9R) XP® Rabbit mAb | Cell Signaling Technology | Cat. No. 70076 |
| Mouse (G3A1) mAb IgG1 Isotype Control | Cell Signaling Technology | Cat. No. 5415 |
| GAPDH Antibody Rabbit Polyclonal | Proteintech | Cat. No. 1094-I-AP |
| MCP-1 Antibody (Mouse Specific) | Cell Signaling Technology | Cat. No. 2029 |
| TLR1 Rat anti-Mouse, Clone: 285923 | R &D systems | Cat. No. MAB1475 |
| TLR2 Goat anti-Mouse, Polyclonal | R &D systems | Cat. No. AF1530 |
| TLR4 Antibody (25) | Santa Cruz Biotechnology | Cat. No. 293072 |
| IL-10 Antibody (E-10) | Santa Cruz Biotechnology | Cat. No. 8438 |
| TLR2 Antibody (TL2.1) | Santa Cruz Biotechnology | Cat. No. 21759 |
| TNF alpha Antibody | Santa Cruz Biotechnology | Cat. No. 52746 |
| Anti-MyD88 antibody | Abcam | Cat. No. ab135693 |
| Anti-IL-10 antibody [JES5-2A5] | Abcam | Cat. No. ab33471 |
| InVivoMAb anti-mouse IL-10R (CD210), clone: 1B1.3A | InVivoMab Antibodies | Cat. No. BE0050 |
| Recombinant Anti-G-CSF antibody [EPR3203(N)(B)] | Abcam | Cat. No. ab181053 |
| TRAF6 Polyclonal Antibody | Invitrogen | Cat. No. 38-0900 |
| GM-CSF Monoclonal Antibody (22E9) | Invitrogen | Cat. No. MM500C |
| IL-10 Monoclonal Antibody (JES3-9D7) | Invitrogen | Cat. No. AHC0102 |
| Mouse (MOPC-21) mAb IgG1 Isotype Control | Cell Signaling Technology | Cat. No. 4097 |
| InVivoMAb anti-mouse IL-10, Clone: JES5-2A5 | InVivoMab Antibodies | Cat. No. BE0049 |
| anti-horseradish peroxidase, Clone: HRPN | InVivoMab Antibodies | Cat. No. BE0088 |
| F4/80 (D2S9R) XP® Rabbit mAb | Cell Signaling Technology | Cat. No. 70076 |
| MMR/CD206/Mannose Receptor Antibody | Novus Biologicals | Cat no. NBP1-90020 |
| iNOS Antibody | Novus Biologicals | Cat no. NBP2-22119 |
| F4/80 Antibody (C-7) | Santa Cruz Biotechnology | Cat no. 377009 |
| Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 | Thermo Fisher | Cat no. A11-012 |
| Alexa Fluor® 488 AffiniPure Goat Anti-Mouse IgG (H+L) | Jackson Immuno Research | Cat no. 115-545-062 |
| Anti-α-SMA antibody | Abcam | Cat. No. ab5694 |
| Anti-vimentin antibody | Abcam | Cat. No. ab92547 |
| Reagents and kits | ||
| Hematoxylin | Thermo Fisher | Cat. No. 7111L |
| Eosin Y | Thermo Fisher | Cat. No. 7211L |
| Bluing Reagent | Thermo Fisher | Cat. No. 7301L |
| Masson’s Trichrome stain | Abcam | Cat. No. ab150686 |
| EasySep Human Monocytes Enrichment Kit | STEMCELL Technologies | Cat. No. 19359 |
| EasySep Mouse monocytes Enrichment Kit | STEMCELL Technologies | Cat. No. 19861 |
| EasySep Buffer | STEMCELL Technologies | Cat. No. 20144 |
| SYBR™ Green PCR Master Mix | Thermo Fisher | Cat. No. 4309155 |
| SuperScript™ III First-Strand Synthesis System | Thermo Fisher | Cat. No. 18080051 |
| Elastic connective tissue stain | Abcam | Cat. No. ab150667 |
| Critical Commercial Assays | ||
| CCL2 ELISA kit | Thermo Fisher | Cat. No. 88-7391-22 |
| IL-1β ELISA kit | Thermo Fisher | Cat. No. 88-7013-88 |
| TNF-α ELISA kit | Thermo Fisher | Cat. No. 88-7324-88 |
| EZCell™ Glucose Uptake Assay kit | BioVision | Cat. No. K924 |
| Experimental Models: Organisms | ||
| Mouse: C57BL/6J | Jackson laboratories | 000664 |
| Mouse: C57BLKS-m leprdb(db/db) | Jackson laboratories | 000642 |
| Oligonucleotides | ||
| Human: | ||
| Tlr2 Forward: GAAGAGTGAGTGGTGCAAGTAT, Reverse: AATGGGCTCCAGAAGAATGAG; | Integrated DNA Technologies | N/A |
| Tlr4 Forward: TTTCAGCTCTGCCTTCACTAC, Reverse: GACACCACAACAATCACCTTTC | Integrated DNA Technologies | N/A |
| MYD88 Forward: CTGTGTCTGGTCTATTGCTAGTG, Reverse: TTCCTTGCTCTGCAGGTAATC | Integrated DNA Technologies | N/A |
| IL1 Forward: CAAAGGCGGCCAGGATATAA, Reverse: CTAGGGATTGAGTCCACATTCAG | Integrated DNA Technologies | N/A |
| TNF Forward: GCAGGTCTACTTTGGGATCATT, Reverse: AGAAGAGGTTGAGGGTGTC | Integrated DNA Technologies | N/A |
| RelA Forward: CTGTCCTTTCTCATCCCATCTT, Reverse: TCCTCTTTCTGCACCTTGTC | Integrated DNA Technologies | N/A |
| RelB Forward: CTGCGGATTTGCCGAATTAAC, Reverse: ACACCACTGATATGTCCTCTTTC | Integrated DNA Technologies | N/A |
| Ccl2 Forward: TCATAGCAGCCACCTTCATTC, Reverse: CTCTGCACTGAGATCTTCCTATTG | Integrated DNA Technologies | N/A |
| Ccl3 Forward: GGCAGATTCCACAGAATTTCATAG, Reverse: TCGCTTGGTTAGGAAGATGAC | Integrated DNA Technologies | N/A |
| IL10 Forward: TCCTTGCTGGAGGACTTTAAGGGT, Reverse: TGTCTGGGTCTTGGTTCTCAGCTT | Integrated DNA Technologies | N/A |
| 18s Forward: CACGGACAGGATTGACAGATT, Reverse: GCCAGAGTCTCGTTCGTTATC | Integrated DNA Technologies | N/A |
| GAPDH Forward: GGTGTGAACCATGAGAAGTATGA, Reverse: GAGTCCTTCCACGATACCAAAG | Integrated DNA Technologies | N/A |
| Mice: | ||
| granulocyte (Csf3) Forward: TGTTCCCAAACTGGGTTCTT, Reverse: TGGCTGCCACTGTTTCTT | Integrated DNA Technologies | N/A |
| GM-CSF Forward: AGCTCTGAATCCAGCTTCTC, Reverse: CCACATCTCTTGGTCCCTTTA | Integrated DNA Technologies | N/A |
| Ccl2 Forward: CTCACCTGCTGCTACTCATTC, Reverse: ACTACAGCTTCTTTGGGACAC | Integrated DNA Technologies | N/A |
| Ccl3 Forward: TCACTGACCTGGAACTGAATG, Reverse: CAGCTTATAGGAGATGGAGCTATG | Integrated DNA Technologies | N/A |
| Rela Forward: CCGACTTGTTTGGGTGATCT, Reverse: TCCGTCTCCAGGAGGTTAAT | Integrated DNA Technologies | N/A |
| Relb Forward: TGCCGAATCAACAAGGAGAG, Reverse: TGCTGAACACCACGGATATG | Integrated DNA technologies | N/A |
| Tlr2 Forward: CACTATCCGGAGGTTGCATATC, Reverse: GGAAGACCTTGCTGTTCTCTAC | Integrated DNA Technologies | N/A |
| Tlr4 Forward: GAGCAAACAGCAGAGGAAGA, Reverse: CCAGGTGAGCTGTAGCATTTA | Integrated DNA Technologies | N/A |
| Myd88 Forward: AGCAACTAGGACTGCCTTTC, Reverse: GAACTCTTCCACTCAGCTATCC | Integrated DNA Technologies | N/A |
| IL-1 Forward: GCACTACAGGCTCCGAGATGAAC; Reverse: TTGTCGTTGCTTGGTTCTCCTTGT | Integrated DNA Technologies | N/A |
| Tnf Forward: TTGTCTACTCCCAGGTTCTCT, Reverse: GAGGTTGACTTTCTCCTGGTATG | Integrated DNA Technologies | N/A |
| GAPDH Forward: TTGGGTTGTACATCCAAGCA, Reverse: CAAGAAACAGGGGAGCTGAG | Integrated DNA Technologies | N/A |
| Cell lines | ||
| Mouse Primary Epidermal Keratinocytes | Cell Biologics | Cat No: C57-6066K |
| Mouse Primary Dermal Lymphatic Endothelial Cells | Cell Biologics | Cat No: C57-6064L |
| Software and Algorithms | ||
| GraphPad Prism | GraphPad | https://graphpad.com/scientific-software/prism/ |