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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Curr Opin Struct Biol. 2022 Jan 5;72:248–259. doi: 10.1016/j.sbi.2021.11.016

Figure 4. ENGase accessory domains that define substrate specificity.

Figure 4.

A. Overall structure of EndoS and EndoS2. B. Hydrogen-deuterium exchange mass spectrometry of EndoS2 and IgG1. Difference in deuteration between unliganded- and IgG1-bound EndoS2E186L (left and center) and IgG1 and EndoS2E186L-bound IgG1 (right), mapped onto a surface representation of EndoS2 (PDB 6MDS) and IgG1 (PDB 1HZH) at the earliest time point tested (10 s). IgG1 glycosylation sites annotated, and Fc, Fab, GH and CBM domains are labeled for orientation. C. Overall structure of EndoBT-3987, EndoA and EndoD.