This figure presents cFos immunoreactivity (IR) of slices to compare neuronal activation between the ipsi and contralateral sides in each stage and activation between stages. Areas with roman numerals in the image are magnified for better visualization of the cFos IR. (A) Ipsilateral cFos IR observed in the anterior cingulate gyrus and primary somatosensory cortex during early focal intermittent seizures (B) Bilateral cFos IR in the anterior cingulate gyrus and somatosensory cortex during continuous generalized convulsive seizures. (C) Ipsilateral cFos IR observed primarily in cortical and amygdalar areas. Sparse cFos immunoreactivity was observed in the thalamus and hippocampus. (D) Bilateral cFos IR observed in the cortical and subcortical areas. There is a striking difference of cFos IR at the hippocampus, especially CA1, CA2 and the dentate gyrus (DG), between both stages. It can also be observed that cortical cFos IR (at the retrosplenial, somatosensory, auditory and piriform cortices) in the late stage was increased from the early stage. In addition, the cFos IR at the amygdala (CIV-V vs DIV-V) was elevated in the late stage compared the early stage and became bilateral.