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. 2022 Feb 15;20:1002–1011. doi: 10.1016/j.csbj.2022.02.009

Fig. 5.

Fig. 5

Transcriptome and RT-qPCR analyses for KGM biosynthesis. (A) Principal component analysis (PCA) of 15 A. konjac corm samples. (B) RT-qPCR and measurement of KGM content. Values represent means ± SD. Asterisks indicate statistical significance using student’s t-test (P < 0.05, n = 3) and one-way ANOVA with post hoc Tukey HSD test is applied to compare KGM content of four stages (P < 0.01, n = 4). (C) Heatmap of KGM biosynthesis-related genes that are highly expressed in stage 2 and/or stage 3. The threshold is Log2 FC (stageN/stage1) > 2 (N = 2 or 3, P < 0.05).