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. 2022 Feb 8;9:816064. doi: 10.3389/fvets.2022.816064

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Copyright © 2022 Yu, Gao, Loor, Jiang, Fang, Hao, Shi, Fan, Chen, Li, Liu, Wang, Li and Du.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcriptional activity of transcription factor EB (TFEB) in white adipose tissue (WAT). (A) Western blot analysis of TFEB in the nucleus and cytoplasm of WAT of control cows (n = 15) and dairy cows with subclinical ketosis (SCK; n = 15). Representative blots are shown. (B,C) Quantification of protein abundance of nuclear and cytoplasmic TFEB, respectively. (D) Western blot analysis of phosphorylated TFEB (p-TFEB) and total TFEB in WAT of control cows (n = 15) and dairy cows with SCK (n = 15). Representative blots are shown. (E,F) Quantification of ratio of p-TFEB/TFEB and protein abundance of TFEB, respectively. (G,H) Relative mRNA abundance of TFEB and peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PPARGC1A) in WAT of control cows (n = 15) and dairy cows with SCK (n = 15). Data were analyzed using unpaired t-tests and expressed as mean ± SEM.