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. 2022 Feb 21;12:2889. doi: 10.1038/s41598-022-06587-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Viability of HCT116 cells transfected with miRNA mimics that show a strong synergetic effect with 2.5 mM metformin treatment. Normalized cell counts of HCT116 cells transfected with miRNA mimics, treated with 2.5 mM metformin and compared with 0 mM metformin treatment. Results are expressed as mean ± SD of 3 replicates and the statistical significance is indicated with asterisks (ns P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 and ****P ≤ 0.0001).