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. 2022 Feb 21;12:2889. doi: 10.1038/s41598-022-06587-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in glycolysis (a), maximum glycolytic capacity (b) and glycolytic reserve (c) associated with miRNA mimic transfections in combination with metformin treatment of HCT116 cells. CRC cells were reverse transfected with selected miRNA mimics and treated with 2.5 mM metformin or control medium. Extracellular acidification rate (ECAR) of the cells was determined over a 30-min period using a Seahorse XFe-96 Instrument. The ECAR values were normalized to viability of the cells per group determined by crystal violet assay. Results are expressed as mean ± SD of 3 culture replicates and the statistical significance is indicated with asterisks (ns P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.