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. 2022 Jan 25;298(3):101641. doi: 10.1016/j.jbc.2022.101641

Figure 4.

Figure 4

AdipoRon treatment promotes adipogenesis and lipolysis in vitro.A, diagram of the AdipoRon treatment regimen during 3T3-L1 adipogenic differentiation. B, representative images of adipogenic differentiation in 3T3-L1 cells. C, relative mRNA levels of Glut4. D, relative mRNA levels of Cide family. E, relative mRNA levels of Pnpla2 and Lipe. F, relative mRNA levels of lipolytic cofactors. G, relative mRNA levels of mitochondrial marker genes. H, representative protein levels of ATGL, p-HSL, total HSL, PPARγ, and CEBPα normalized to that of GAPDH. I and J, quantification of lipolysis-related and abiogenesis-related protein levels. K, diagram of coculture of primary adipocyte–derived mature adipocytes and muscle satellite cell–derived myofibers. Briefly, primary adipocytes and muscle satellite cells were seeded in the bottom and top wells of a transwell system, respectively, followed by adipogenic differentiation with DMSO, AdipoRon, or AdipoRon + anti-FGF21 antibody and myogenic differentiation. Then, the layers were resembled in a new coculture system. L, FGF21 concentrations in medium. M, relative contents of Oil Red O in myofibers. N, representative images of Oil Red O staining. O, relative contents of Oil Red O in differentiated 3T3-L1 cells. Data are presented as the mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. ATGL, adipose triglyceride lipase; CEBPα, CCAAT/enhancer-binding protein alpha; DMSO, dimethyl sulfoxide; p-HSL, phosphorylated hormone-sensitive lipase; PPARγ, peroxisome proliferator–activated receptor alpha.