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. 2022 Feb 21;13(2):167. doi: 10.1038/s41419-022-04561-x

Fig. 5. circNEIL3 regulates PIF1 expression via directly binding miR-1184.

Fig. 5

a RNA immunoprecipitation performed using AGO2 antibody in A549 cells transfected with miR-1184 mimics or negative control (NC) mimics, followed by the detection of circNEIL3 enrichment. b Wild-type (WT) and mutant (Mut) transcripts of circNEIL3 or the PIF1 3′-untranslated region (UTR) for luciferase reporters. c, d Dual luciferase reporter activity of circNEIL3 c and the PIF1 3′-UTR d in A549 cells co-transfected with either miR-1184 mimics or NC mimics. e, f Dual luciferase reporter activity of the PIF1 3′-UTR in A549 cells with circNEIL3 overexpression e or knockdown f. g Expression of PIF1 in LUAD cells transfected with miR-1184 mimics alone or co-transfected with circNEIL3. h Expression of PIF1 in LUAD cells transfected with miR-1184 inhibitor alone or co-transfected with si-circNEIL3. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student’s t-test.