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. 2022 Feb 21;13(2):167. doi: 10.1038/s41419-022-04561-x

Fig. 6. The circNEIL3/miR-1184/PIF1 axis mediates pyroptosis via the DNA damage repair pathway.

Fig. 6

a–c Enzyme-linked immunosorbent assays detecting the release of LDH, IL-1β, and IL-18 in LUAD cells transfected with miR-1184 mimics alone or co-transfected with circNEIL3. d Western blot of the expression of active-caspase-1 and GSDMD-N in the indicated groups. e Expression levels of γH2AX and BRCA1 in irradiated LUAD cells with circNEIL3 overexpression. f Immunofluorescence staining of γH2AX in A549 cells with or without circNEIL3 overexpression. g Expression levels of γH2AX and BRCA1 in irradiated LUAD cells with PIF1 knockdown. h Immunofluorescence staining of γH2AX in A549 cells treated with si-nc or si-PIF1. i Immunofluorescence co-staining of γH2AX and ASC in A549 cells treated with si-nc or si-PIF1. Scale bar, 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student’s t-test. Con, control; IR, irradiated.