Figure 3.

DJ-1 and Nrf2/GPX4 signal pathway expression levels in placental tissue from normal and preeclamptic pregnancies. (A,F,I) In reverse transcriptase-polymerase chain reaction (RT-PCR), expression level of DJ-1, Nrf2 and GPX4 mRNA was significantly increased in PE group (P₁ = 0.005, P₂ = 0.022, P₃ = 0.029). (B,C,G,H,J,K) In Western Blot, expression level of DJ-1, Nrf2 and GPX4 protein was significantly increased in PE group (P₁ = 0.005, P₂ < 0.001, P₃ < 0.001). (D,E) In immunohistochemistry, expression level of DJ-1 protein was significantly increased in PE group (p < 0.001). Comparison between two groups was conducted by the independent sample t-test.