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The MDA reaction system.
| Standard tube | Blank tube | Test tube | Control tube | |
|---|---|---|---|---|
| 10 nmol/ml standard substance (ml) | a | |||
| Absolute ethanol (ml) | a | |||
| Test sample (ml) | a | a | ||
| Reagent1 (ml) | a | a | a | a |
| Mixing | ||||
| Reagent2 (ml) | 3 | 3 | 3 | 3 |
| Reagent3 (ml) | 1 | 1 | 1 | |
| 50% glacial acetic acid (ml) | 1 | |||
