Table 3.
qRT-PCR reaction system.
| Reagent | Volume(μL) |
|---|---|
| SYBR Premix Ex Taq | 30.0 |
| PCR Forward Primer (10 μM) | 1.2 |
| PCR Reverse Primer (10 μM) | 1.2 |
| cDNA (< 100 ng) | 6.0 |
| ROX Reference Dye (50 ×) | 1.2 |
| dH2O | 20.4 |
| Total | 60.0 |
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