FIG. 2.

Western blot analysis of H. canis gamont antigens recognized by sera from dogs infected with H. canis and H. americanum. Reactions of sera from different dogs are shown as follows: lane 1, serum from a dog experimentally infected with H. canis; lane 2, serum from a dog with a naturally occurring H. canis infection; lanes 3 to 5, sera from three dogs that were naturally infected with H. americanum; lanes 6 and 7, control serum from a blood donor dog at Auburn University and from preinfection serum from the experimentally infected dog, respectively. Lane M contains molecular mass markers. Note the triplet of bands with relative rMM of 32 to 28 kDa that are reactive only with anti-H. canis sera.