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. 2022 Feb 8;8:792322. doi: 10.3389/fvets.2021.792322

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Copyright © 2022 Cao, Tang, Zhang, Li, Li, Tian and Xu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Response surface plots for FCV. (A) 3D response graphs based on different combinations of primer concentration, probe concentration, and annealing temperature generated by computer-aided exchange procedure. (B) Response surface graphs of primer concentration and annealing temperature at different probe concentrations.